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Micro-flow Processor for Microarrays & Membranes
  • Overview
  • Quick View Leaflet
  • Detail Technical Brochure
  • Application Procedure
Micro-flow Processor for Microarrays & Membranes
Overview

Hybridization is a routinely used method in molecular biology laboratories. This procedure involves the treatment of a substrate, generally a membrane or a glass slide with different buffers and biochemical reagents. The whole process involves about 12 to 15 steps and takes about three to four hours based on the protocol. The Micro-flow Processor is controlled through a PC and permits the user to configure the various steps in post-hybridization treatment. This implementation has the advantages of improved repeatability across experiments and at the same time processing can be carried out by individuals with minimum technical knowledge. It can also be used in processes that require the controlled and calibrated flow of multiple reagents in the sub-milliliter range.

Micro-flow Processor for Microarrays & Membranes
Quick View Leaflet

Hybridization is a routinely used method in molecular biology laboratories. This procedure involves the treatment of a substrate, generally a membrane or a glass slide with different buffers and biochemical reagents. The whole process involves about 12 to 15 steps and takes about three to four hours based on the protocol. The Micro-flow Processor is controlled through a PC and permits the user to configure the various steps in post-hybridization treatment. This implementation has the advantages of improved repeatability across experiments and at the same time processing can be carried out by individuals with minimum technical knowledge.

ADVANTAGES

  • Programmable Parameters:

    1. Selection between two reaction chambers / sample holders.
    2. Selection of any one of the eight reagents for pumping through the reaction chamber.
    3. Flow-rate control in the range of ~10 to 40 µl per second.

  • User interface for defining a sequence of steps defining the above parameters (i.e., reagent selection, flow-rate / flow-volume and set temperature).

  • The temperature of two reagents can be maintained from 40 to 60 (+/- 0.5) ° C.

  • The system interfaces to a PC through or serial port.


APPLICATIONS

  • Post hybridization Processing of microarray and other membranes.

  • It can also be used in processes that require the controlled and calibrated flow of multiple reagents in the sub-milliliter range.
Micro-flow Processor for Microarrays & Membranes
Detail Technical Brochure


Hybridization is a routinely used method in molecular biology. This procedure involves the treatment of a substrate, generally a membrane or a glass slide with different buffers and biochemical reagents. The whole process involves multiple steps and takes a few hours based on the protocol.

The Micro-flow Processor is controlled through a microprocessor or a PC and permits the user to configure the various steps in post-hybridization treatment. This implementation has the advantages of improved repeatability across experiments and at the same the processing can be carried out by individuals with minimum technical knowledge. It can also be used in processes that require the controlled and calibrated flow of multiple reagents in the sub-milliliter range.

MAIN COMPONENTS OF THE SYSTEM

  • The system consists of three motorized multi-port valves and one peristaltic pump with the necessary control electronics.
  • Two independently selectable reaction chambers that accommodate one slide each of 25 mm X 25 mm X 1 mm dimensions.
  • Reagent holder for holding standard laboratory tubes / bottles in the range of 50 to 0.5 ml.
  • Reagent holder with a provision for heating for holding two bottles of 20 ml each.
  • GUI based software interface for
    • Defining / Storing and Recalling user defined methods.
    • Manual operation of the valves and the pump.

TECHNICAL SPECIFICATIONS

  1. Programmable Parameters:

    1. Selection between two reaction chambers / sample holders.
    2. Selection of any one of the eight reagents for pumping through the reaction chamber.
    3. Flow-rate control in the range of ~10 to 40 µl per second.

  2. User interface for defining a sequence of steps defining the above parameters (i.e., reagent selection, flow-rate / flow-volume and set temperature).

  3. The temperature of two reagents can be maintained from 40 to 60 (+/- 0.5) ° C.

  4. The system interfaces to a PC through either USB or serial port.

  5. Dimensions (mm) [WxHxD] : 420*300*220 ; Weight: ~5Kg.

  6. Hardware Required : PC (Pentium IV or above), min 512 MB-RAM, Windows-XP-SP3

INFRASTRUCTURE

  • An electronic workshop having PCB assembly, SMD workbench & Instrument rack wiring facility.
  • A PC with Windows XP or Windows 7 Operating System.
  • 230 Volts, 5 Ampere, Single Phase Power supply.
  • Access to a mechanical workshop having lathe and sheet bending machine.

Manpower :

  • One person having Diploma in Electronics Engineering and one semi-skilled worker.

Space :

  • One work bench equivalent space (4”X6”) is required for assembly and testing

APPLICATIONS

  • Post hybridization Processing of microarray and other membranes.

  • It can also be used in processes that require the controlled and calibrated flow of multiple reagents in the sub-milliliter range.

 

Who Should apply


Interested parties with Engineering & Scientific knowledge, good financial background and adequate experience of products manufacturing & fabrication with technical capability in the area of interested technology and having or interested in setting –up facilities for production would be preferred.


How to apply

Send your Technology Transfer Application form duly filled and signed alongwith a Demand Draft/Bankers cheque of Rs. 500/- (for Indian entities) or US $50/- (for foreign entities) drawn in favour of “Accounts Officer, BARC” as application processing fee on following address: 

Head, Technology Transfer and Collaboration Division 
Bhabha Atomic Research Centre,
Trombay, Mumbai – 400 085, India.

Fax : +91-22-25505151


Note:Applications without processing fee as applicable above of Rs. 500/- or US $50/- for each technology will not be considered.


Click here for Technology Transfer Application Form

Contact Us

Head, 
Technology Transfer and Collaboration Division
Bhabha Atomic Research Centre,
Trombay, Mumbai 400 085.

Tel  : 091-022-25595137
Fax : 091-022- 25505151
Email : technology@barc.gov.in

How to apply

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