(`AKRUTI Tech Pack’)
Offered by Bhabha Atomic Research Centre
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TECH PACK TECHNOLOGY: Banana Tissue Culture
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BANANA TISSUE CULTURE

BTC

INTRODUCTION

Banana and plantain (Musa spp.) are the most important fruit crops of India; the largest banana producer on the globe.

The major problem however faced in the banana growing areas is the shortage of uniform and disease-free planting materials, which is due to the vegetative mode of propagation of the plant (through side-suckers) at a slow pace in conventional cultivation.

This difficulty can be addressed by employing the commercial tissue culture technology for mass-production of the popular banana varieties.

The said tissue culture technology can also be utilized for conservation & multiplication (as per the requirement) of other locally important, elite, endangered and ornamental banana varieties.

GENERAL STEPS INVOLVED

The banana tissue culture process involves

  • Initiation of aseptic cultures from shoot-tips obtained from disease-free banana plants.
  • Multiplication of the shoots to the desired scale.
  • Induction of roots to individual shoots.
  • Primary & secondary hardening of the tender plantlets in the poly-house.
  • Field-transfer of the hardened tissue culture plants.

ADVANTAGES

BTC The ‘banana tissue culture propagation’ is now an established and popular technology due to the advantages it offers such as : large-scale production of disease-free planting materials, comparatively uniform plant-stand in the field, early harvesting of superior fruit bunches, round the year availability of the planting materials etc.

 

BARC has developed and transferred the banana tissue culture know-how for large and` small scale commercial production to the users in semi-urban and rural India; including the small scale tissue culture laboratory established for AKRUTI-CARD at Anjangaon-Surji, district Amravati, Maharashtra.

 

The spread and popularization of “BARC’s Banana Tissue Culture Know-how” at grass-root level opens up new avenues for sustainable developments in rural India.

 

REQUIREMENTS

Infrastructure : A tissue culture laboratory, a poly-house facility for weaning tissue culture plantlets.
Equipments : Standard glassware, tissue culture racks, clean-air bench, autoclave, pH meter, weighing balances, surgical-ware etc.
Chemicals : All the ingredients of Murashige & Skoog’s basal medium, sucrose, growth hormones, gelling agents etc.
Manpower : Skilled technician/s (trained in a tissue culture laboratory) for laboratory work and supporting staff & workers.

 

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